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Product Info Summary
| SKU: | LS054764 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of zinc finger, RAN-binding domain containing 1 (ZRANB1) |
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Product info
Product Name
ZRANB1 (NM_017580) Human Over-expression Lysate
See all ZRANB1/Trabid products
SKU/Catalog Number
LS054764
Size
100 µg
Description
Transient overexpression lysate of zinc finger, RAN-binding domain containing 1 (ZRANB1)
Storage & Handling
Cite This Product
ZRANB1 (NM_017580) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS054764)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ZRANB1 Human Over-expression Lysates NM_017580.
Western blot validation of overexpression lysate (LS054764) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZRANB1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ZRANB1 (Source: Uniprot.org, NCBI)
Gene Name
ZRANB1
Full Name
Ubiquitin thioesterase ZRANB1
Weight
80.967kDa
Superfamily
peptidase C64 family
Alternative Names
HRPE773; jacalin-like lectin domain containing 2; JCLN2; pancreatic adenocarcinoma upregulated factor; PAUF; PRO1567; Trabid; ZRANB1; zymogen granule protein 16 homolog B (rat); zymogen granule protein 16 homolog B ZRANB1 TRABID zinc finger RANBP2-type containing 1 ubiquitin thioesterase ZRANB1|TRAF-binding domain-containing protein|TRAF-binding protein domain|hTrabid|zinc finger Ran-binding domain-containing protein 1|zinc finger, RAN-binding domain containing 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ZRANB1, check out the ZRANB1 Infographic
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In this infographic, you will see the following information for ZRANB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ZRANB1 (NM_017580) Human Over-expression Lysate (LS054764)
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